150000001875 compounds Chemical class 0.000 claims description 89.230000004044 response Effects 0.000 claims abstract description 45.Priority claimed from PCT/US2017/039609 external-priority patent/WO2018005569A1/en Application granted granted Critical Publication of US9990350B2 publication Critical patent/US9990350B2/en Status Active legal-status Critical Current Anticipated expiration legal-status Critical Links Assignors: OTERO, Carlos Augusto, CAMPBELL, JOHN, RADAKOVITZ, SAMUEL C., FICHTNER, MATTHEW HART, CANTON, Christian, RAMPSON, BENJAMIN EDWARD Publication of US20170124048A1 publication Critical patent/US20170124048A1/en Assigned to MICROSOFT TECHNOLOGY LICENSING, LLC reassignment MICROSOFT TECHNOLOGY LICENSING, LLC ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority to US201562249884P priority Critical Priority to US201562249869P priority Priority to US201662357363P priority Priority to US201662357284P priority Priority to US201662357292P priority Application filed by Microsoft Technology Licensing LLC filed Critical Microsoft Technology Licensing LLC Priority to US15/282,269 priority patent/US9990350B2/en Assigned to MICROSOFT TECHNOLOGY LICENSING, LLC reassignment MICROSOFT TECHNOLOGY LICENSING, LLC ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Original Assignee Microsoft Technology Licensing LLC Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) Radakovitz Benjamin Edward Rampson Current Assignee (The listed assignees may be inaccurate. Campbell Carlos Augusto Otero Christian Canton Matthew Hart Fichtner Samuel C. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Active Application number US15/282,269 Other versions US20170124048A1
Google Patents Videos associated with cells in spreadsheetsĭownload PDF Info Publication number US9990350B2 US9990350B2 US15/282,269 US201615282269A US9990350B2 US 9990350 B2 US9990350 B2 US 9990350B2 US 201615282269 A US201615282269 A US 201615282269A US 9990350 B2 US9990350 B2 US 9990350B2 Authority US United States Prior art keywords data cell video spreadsheet attributes Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents US9990350B2 - Videos associated with cells in spreadsheets Cells could be observed during migration through the agarose gel (black part, wall).US9990350B2 - Videos associated with cells in spreadsheets Media only was poured in the left well as negative control and BMHC were seeded in the right well. MDA-MB231 cells were seeded in the central well. BMHC were able to increase proliferation of MDA-MB231. MDA-MB231 were plated and counted every 2 days in presence or not of BMHC during 6 days. HBMEC (human bone marrow endothelial cells) or plastic were used as negative control. BMHC were plated up to 60 % confluency, 50,000 eGFP MDA-MB231 were allowed to adhere for 1 h. e Adhesion assay testing the specificity of the adhesion between MDA-MB231 cells and BMHC. Z-X reconstitution shows that cancer cells (green) are growing on BMHC. Before imaging by confocal microscopy, co-cultures were stained with Alexa Fluor 594 conjugated-wheat germ agglutinin (WGA). BMHC were co-cultured with tumor cells for 3 days. d Confocal imaging of BMHC and eGFP MDA-MB231 co-culture.
c Co-culture of BMHC and MDA-MB231 in phase microscopy. Very close interaction between the two cellular membranes of MCF7 and BMHC can be observed with formation of tight junction ( right panel, arrows). A pseudopodia of BMHC with two MDA-MB-231 cells were closely interacting with the pseudopodia ( left panel, arrows). MDA-MB-231 and BMHC or MCF7 and BMHC were co-cultured during 48 h and analyzed by electronic microscopy. The insert picture showed the metastatic node the tissue micro-array.
Picture showed a network of BMHC (brown cells) surrounding cancer cell clusters (blue cells). a Paraffin-embedded immunohistochemistry. Intercellular interactions between cancer cells and Bone Marrow Host Cells (BMHC).